Among those slated for deportation are 13 children, the group alleged.
The group called upon the United Nations Refugee Agency (UNHCR) and the international community to intervene to avoid their forced expulsion.
The Eritrean government is among the worst human rights violators in the world.
President Isaias Afeworki who has led the country since it gained independence from Ethiopia in 1993 has no tolerance for dissent.
If any citizen is suspected of being a political threat to the regime the individual is thrown in jail indefinitely.
Following the 1998-2000 war with Ethiopia over border dispute, Eritrean authorities have arbitrarily detained thousands who most remain held without charge.
The flashpoint own of Badme was awarded to Eritrea by the Eritrea-Ethiopia Boundary Commission but Ethiopia refused to implement what was supposed to be a "final and binding."
Eritrea, which is described as "a giant prison" by Human Rights Watch (HRW), currently has an estimated 10,000 political prisoners locked at the country's appalling secret detention centers.