At least seven people were burnt beyond recognition and died after the taxi they were travelling in crashed into a truck along the N1 in Limpopo on Sunday morning.
The provincial health department has confirmed that a forensics team is now combing through the wreckage.
The highway, between Kranskop and Mookgophong, would be closed to allow the officials to work, provincial transport and community safety department spokesperson Matome Moremi-Tauatsoala said.
In September, over 40 people died in separate accidents within a 20km radius of the current scene.
Those who perished include 11 Malawian tourists, who were on their way to Durban when their bus was involved in an accident.
Last week, Transport Minister Blade Nzimande announced a R4bn investment to improve the N1 between the Kranskop toll plaza and Makhado.
Speaking during the launch of the festive season road safety campaign in Modimolle, Nzimande had visited a section of the N1 along with officials and religious leaders to cleanse the road, which is notorious for fatal crashes.